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2-well chamber slides with glass coverslip bottoms  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 2-well chamber slides with glass coverslip bottoms
    2 Well Chamber Slides With Glass Coverslip Bottoms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2-well+glass+chamber+slides/2+well+chamber+slides+with+glass+coverslip+bottoms/pmc11228511__pnas__2406946121__sapp-55-14-17
    Average 90 stars, based on 1 article reviews
    2-well chamber slides with glass coverslip bottoms - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunocytochemistry:

    Article Title: Identification and Structural Determination of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal
    Article Snippet: Transfection and Immunocytochemical Analysis—MDCK cells were seeded on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International, Naperville, IL).

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) under identical experimental conditions as described for cultures meant for biochemical analysis, though the seeding density and treatment doses were adjusted proportionally to the substrate surface area and the cell number respectively.

    Article Title: Identification of a Novel Apical Sorting Motif and Mechanism of Targeting of the M2 Muscarinic Acetylcholine Receptor
    Article Snippet: Cholera Toxin and Antibody-induced Patching—MDCK cells stably expressing FLAG-tagged M2 mAChR were plated on MDCK cells seeded at 3.5 105 cells/well on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International) and grown to confluency for 3–4 days.

    Article Title: N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.
    Article Snippet: Explants were cultured on 2-well glass chamber slides (ThermoFisher) coated with Poly-L-Lysine (0.01% PLL, Sigma) and fibronectin (0.01%, Sigma).

    Article Title: Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors.
    Article Snippet: Background: Bone-to-tendon healing after rotator cuff repairs is mainly impaired by poor tissue quality.. The tenocytes of chronic rotator cuff tendon tears are not able to synthesize normal fibrocartilaginous extracellular matrix (ECM).. We hypothesized that in the presence of platelet-released growth factors (PRGF), tenocytes from chronically retracted rotator cuff tendons proliferate and synthesize the appropriate ECM proteins.

    Article Title: Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells.
    Article Snippet: Briefly, cells were seeded at a density of 300,000 cells/chamber in 2-well glass chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) and were treated for 24 h with silica coated IONPs 100 μg/mL.

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) at a low cell density overnight.

    Cell Culture:

    Article Title: Identification and Structural Determination of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal
    Article Snippet: Transfection and Immunocytochemical Analysis—MDCK cells were seeded on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International, Naperville, IL).

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) under identical experimental conditions as described for cultures meant for biochemical analysis, though the seeding density and treatment doses were adjusted proportionally to the substrate surface area and the cell number respectively.

    Article Title: Identification of a Novel Apical Sorting Motif and Mechanism of Targeting of the M2 Muscarinic Acetylcholine Receptor
    Article Snippet: Cholera Toxin and Antibody-induced Patching—MDCK cells stably expressing FLAG-tagged M2 mAChR were plated on MDCK cells seeded at 3.5 105 cells/well on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International) and grown to confluency for 3–4 days.

    Article Title: N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.
    Article Snippet: Explants were cultured on 2-well glass chamber slides (ThermoFisher) coated with Poly-L-Lysine (0.01% PLL, Sigma) and fibronectin (0.01%, Sigma).

    Article Title: Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors.
    Article Snippet: Background: Bone-to-tendon healing after rotator cuff repairs is mainly impaired by poor tissue quality.. The tenocytes of chronic rotator cuff tendon tears are not able to synthesize normal fibrocartilaginous extracellular matrix (ECM).. We hypothesized that in the presence of platelet-released growth factors (PRGF), tenocytes from chronically retracted rotator cuff tendons proliferate and synthesize the appropriate ECM proteins.

    Article Title: Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells.
    Article Snippet: Briefly, cells were seeded at a density of 300,000 cells/chamber in 2-well glass chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) and were treated for 24 h with silica coated IONPs 100 μg/mL.

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) at a low cell density overnight.

    Stable Transfection:

    Article Title: Identification and Structural Determination of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal
    Article Snippet: Transfection and Immunocytochemical Analysis—MDCK cells were seeded on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International, Naperville, IL).

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) under identical experimental conditions as described for cultures meant for biochemical analysis, though the seeding density and treatment doses were adjusted proportionally to the substrate surface area and the cell number respectively.

    Article Title: Identification of a Novel Apical Sorting Motif and Mechanism of Targeting of the M2 Muscarinic Acetylcholine Receptor
    Article Snippet: Cholera Toxin and Antibody-induced Patching—MDCK cells stably expressing FLAG-tagged M2 mAChR were plated on MDCK cells seeded at 3.5 105 cells/well on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International) and grown to confluency for 3–4 days.

    Article Title: N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.
    Article Snippet: Explants were cultured on 2-well glass chamber slides (ThermoFisher) coated with Poly-L-Lysine (0.01% PLL, Sigma) and fibronectin (0.01%, Sigma).

    Article Title: Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors.
    Article Snippet: Background: Bone-to-tendon healing after rotator cuff repairs is mainly impaired by poor tissue quality.. The tenocytes of chronic rotator cuff tendon tears are not able to synthesize normal fibrocartilaginous extracellular matrix (ECM).. We hypothesized that in the presence of platelet-released growth factors (PRGF), tenocytes from chronically retracted rotator cuff tendons proliferate and synthesize the appropriate ECM proteins.

    Article Title: Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells.
    Article Snippet: Briefly, cells were seeded at a density of 300,000 cells/chamber in 2-well glass chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) and were treated for 24 h with silica coated IONPs 100 μg/mL.

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) at a low cell density overnight.

    Expressing:

    Article Title: Identification and Structural Determination of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal
    Article Snippet: Transfection and Immunocytochemical Analysis—MDCK cells were seeded on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International, Naperville, IL).

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) under identical experimental conditions as described for cultures meant for biochemical analysis, though the seeding density and treatment doses were adjusted proportionally to the substrate surface area and the cell number respectively.

    Article Title: Identification of a Novel Apical Sorting Motif and Mechanism of Targeting of the M2 Muscarinic Acetylcholine Receptor
    Article Snippet: Cholera Toxin and Antibody-induced Patching—MDCK cells stably expressing FLAG-tagged M2 mAChR were plated on MDCK cells seeded at 3.5 105 cells/well on 2-well glass chamber slides (4.2 cm2/well; Nalge Nunc International) and grown to confluency for 3–4 days.

    Article Title: N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.
    Article Snippet: Explants were cultured on 2-well glass chamber slides (ThermoFisher) coated with Poly-L-Lysine (0.01% PLL, Sigma) and fibronectin (0.01%, Sigma).

    Article Title: Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors.
    Article Snippet: Background: Bone-to-tendon healing after rotator cuff repairs is mainly impaired by poor tissue quality.. The tenocytes of chronic rotator cuff tendon tears are not able to synthesize normal fibrocartilaginous extracellular matrix (ECM).. We hypothesized that in the presence of platelet-released growth factors (PRGF), tenocytes from chronically retracted rotator cuff tendons proliferate and synthesize the appropriate ECM proteins.

    Article Title: Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells.
    Article Snippet: Briefly, cells were seeded at a density of 300,000 cells/chamber in 2-well glass chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) and were treated for 24 h with silica coated IONPs 100 μg/mL.

    Article Title: Impact of Pre-Existing Elastic Matrix on TGF-?1 and HA Oligomer-Induced Regenerative Repair by Rat Aortic Smooth Muscle Cells
    Article Snippet: RASMCs were cultured in sterile, 2-well glass chamber slides (Nalge Nunc International, Naperville, IL) at a low cell density overnight.



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    Thermo Fisher lab-tek 2-well glass chamber slides
    a Representative brightfield images of 4T1 Nup210 KO cells on type I collagen and fibronectin. Scale bar = 100 μm. b Representative images of p-FAK (Y397) focal adhesions and F-actin in sg-Ctrl and Nup210 KO cells on type I collagen and fibronectin. Scale bar = 10 μm. c Quantification of focal adhesion area, count, and cell spreading in sg-Ctrl and Nup210 KO 4T1 cells. ANOVA with Tukey’s multiple comparison correction. The box represents the 25th to 75th percentile, whiskers represent data range, and the horizontal line represents the median. d (Left) <t>Immunostaining</t> of Nup210 KO (KO-N13) 4T1 primary tumors with p-FAK Y397 and pan-cytokeratin (Pan-CK) antibodies. Scale bar = 50 μm. (Right) quantification of p-FAK Y397 signal in the Pan-CK-stained tumor area. N = 5 mice per condition, ~10 independent fields per mouse tumor section. Mann–Whitney U test, median with interquartile range. e Western blot of myc-tagged FAK overexpression in Nup210 KO 4T1 cells. f Primary tumor weight in FAK-overexpressing Nup210 KO (KO-N13) 4T1 cells. ANOVA with Tukey’s multiple comparison correction, mean ± s.d. n = 10 mice per group. g Representative lung images of the mice injected with FAK-overexpressing Nup210 KO cells. h Lung metastasis count and metastases normalized to tumor weight in FAK-overexpressing Nup210 KO cells. ANOVA with Tukey’s multiple comparison correction, mean ± s.d. n = 10 mice per group. i Metastasis incidence in mice injected with FAK-overexpressing Nup210 KO cells. χ 2 test with Bonferroni correction. n = 10 mice per group.
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    Image Search Results


    a Representative brightfield images of 4T1 Nup210 KO cells on type I collagen and fibronectin. Scale bar = 100 μm. b Representative images of p-FAK (Y397) focal adhesions and F-actin in sg-Ctrl and Nup210 KO cells on type I collagen and fibronectin. Scale bar = 10 μm. c Quantification of focal adhesion area, count, and cell spreading in sg-Ctrl and Nup210 KO 4T1 cells. ANOVA with Tukey’s multiple comparison correction. The box represents the 25th to 75th percentile, whiskers represent data range, and the horizontal line represents the median. d (Left) Immunostaining of Nup210 KO (KO-N13) 4T1 primary tumors with p-FAK Y397 and pan-cytokeratin (Pan-CK) antibodies. Scale bar = 50 μm. (Right) quantification of p-FAK Y397 signal in the Pan-CK-stained tumor area. N = 5 mice per condition, ~10 independent fields per mouse tumor section. Mann–Whitney U test, median with interquartile range. e Western blot of myc-tagged FAK overexpression in Nup210 KO 4T1 cells. f Primary tumor weight in FAK-overexpressing Nup210 KO (KO-N13) 4T1 cells. ANOVA with Tukey’s multiple comparison correction, mean ± s.d. n = 10 mice per group. g Representative lung images of the mice injected with FAK-overexpressing Nup210 KO cells. h Lung metastasis count and metastases normalized to tumor weight in FAK-overexpressing Nup210 KO cells. ANOVA with Tukey’s multiple comparison correction, mean ± s.d. n = 10 mice per group. i Metastasis incidence in mice injected with FAK-overexpressing Nup210 KO cells. χ 2 test with Bonferroni correction. n = 10 mice per group.

    Journal: Nature Communications

    Article Title: Nuclear pore protein NUP210 depletion suppresses metastasis through heterochromatin-mediated disruption of tumor cell mechanical response

    doi: 10.1038/s41467-021-27451-w

    Figure Lengend Snippet: a Representative brightfield images of 4T1 Nup210 KO cells on type I collagen and fibronectin. Scale bar = 100 μm. b Representative images of p-FAK (Y397) focal adhesions and F-actin in sg-Ctrl and Nup210 KO cells on type I collagen and fibronectin. Scale bar = 10 μm. c Quantification of focal adhesion area, count, and cell spreading in sg-Ctrl and Nup210 KO 4T1 cells. ANOVA with Tukey’s multiple comparison correction. The box represents the 25th to 75th percentile, whiskers represent data range, and the horizontal line represents the median. d (Left) Immunostaining of Nup210 KO (KO-N13) 4T1 primary tumors with p-FAK Y397 and pan-cytokeratin (Pan-CK) antibodies. Scale bar = 50 μm. (Right) quantification of p-FAK Y397 signal in the Pan-CK-stained tumor area. N = 5 mice per condition, ~10 independent fields per mouse tumor section. Mann–Whitney U test, median with interquartile range. e Western blot of myc-tagged FAK overexpression in Nup210 KO 4T1 cells. f Primary tumor weight in FAK-overexpressing Nup210 KO (KO-N13) 4T1 cells. ANOVA with Tukey’s multiple comparison correction, mean ± s.d. n = 10 mice per group. g Representative lung images of the mice injected with FAK-overexpressing Nup210 KO cells. h Lung metastasis count and metastases normalized to tumor weight in FAK-overexpressing Nup210 KO cells. ANOVA with Tukey’s multiple comparison correction, mean ± s.d. n = 10 mice per group. i Metastasis incidence in mice injected with FAK-overexpressing Nup210 KO cells. χ 2 test with Bonferroni correction. n = 10 mice per group.

    Article Snippet: For focal adhesion immunostaining, Lab-Tek 2-well glass chamber slides (Thermo Fisher Scientific) were coated with 50 μg/ml of collagen type I (Gibco) or 20 μg/ml fibronectin.

    Techniques: Comparison, Immunostaining, Staining, MANN-WHITNEY, Western Blot, Over Expression, Injection